Spectroscopic Analysis of Tempeh Protein Content during the Production Process
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Abstract
Tempeh is a local Indonesian food that is liked by the community, both locally and internationally. The high protein content is the reason for choosing tempeh as a complementary food. In its production, tempeh is made by two important processes, including treatment and fermentation. During this process, the protein can be damaged, resulting in a decrease in the protein content of tempeh. Based on these problems, this study aims to analyze the reduction of tempeh protein content during the production process. The analysis used two spectroscopic methods, including FTIR and UV-Visible. FTIR spectroscopy was used to observe the reduction in total protein content, while UV-Vis spectroscopy was used to observe the reduction in dissolved protein content. The results of the study for FTIR analysis showed that at the pretreatment and fermentation stages, both caused a decrease in the total protein content of tempeh. This was observed with a decrease in the intensity of IR absorption at a wavenumber of 1745 cm-1 which is identical to the C=O group and 1543 cm-1 which is identical to the N-H group. Based on the results of the UV-Visible study, it shows that the fermentation time affects the amount of dissolved protein. This situation is supported by the increasing pH of tempeh and decreasing water content during fermentation.
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References
Acković, L. L., Zeković, I., Dramićanin, T., Bro, R., & Dramićanin, M. D. (2018). Modeling food fluorescence with PARAFAC. In Reviews in Fluorescence 2017 (pp. 161–197). Springer.
Ahnan‐Winarno, A. D., Cordeiro, L., Winarno, F. G., Gibbons, J., & Xiao, H. (2021). Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability. Comprehensive Reviews in Food Science and Food Safety, 20(2), 1717–1767.
Babu, P. D., Bhakyaraj, R., & Vidhyalakshmi, R. (2009). A low cost nutritious food “tempeh”-a review. World J Dairy Food Sci, 4(1), 22–27.
Baeten, V., & Dardenne, P. (2002). Spectroscopy: Developments in instrumentation and analysis. Grasas y Aceites, 53(1), 45–63.
Candoğan, K., Altuntas, E. G., & İğci, N. (2021). Authentication and quality assessment of meat products by fourier-transform infrared (FTIR) spectroscopy. Food Engineering Reviews, 13(1), 66–91.
Capitani, D., Sobolev, A. P., Di Tullio, V., Mannina, L., & Proietti, N. (2017). Portable NMR in food analysis. Chemical and Biological Technologies in Agriculture, 4(1), 1–14.
Chang, C., Hsu, C., Chou, S., Chen, Y., Huang, F., & Chung, Y. (2009). Effect of fermentation time on the antioxidant activities of tempeh prepared from fermented soybean using Rhizopus oligosporus. International Journal of Food Science & Technology, 44(4), 799–806.
Churchill, H., Teng, H., & Hazen, R. M. (2004). Correlation of pH-dependent surface interaction forces to amino acid adsorption: Implications for the origin of life. American Mineralogist, 89(7), 1048–1055.
da Silva-Buzanello, R. A., Ferro, A. C., Bona, E., Cardozo-Filho, L., de Araújo, P. H. H., Leimann, F. V., & Gonçalves, O. H. (2015). Validation of an Ultraviolet–visible (UV–Vis) technique for the quantitative determination of curcumin in poly (l-lactic acid) nanoparticles. Food Chemistry, 172, 99–104.
Damanik, R. N. S., Pratiwi, D. Y. W., Widyastuti, N., Rustanti, N., Anjani, G., & Afifah, D. N. (2018). Nutritional composition changes during tempeh gembus processing. IOP Conference Series: Earth and Environmental Science, 116(1), 12026.
Domingues, D. S., Pauli, E. D., de Abreu, J. E. M., Massura, F. W., Cristiano, V., Santos, M. J., & Nixdorf, S. L. (2014). Detection of roasted and ground coffee adulteration by HPLC by amperometric and by post-column derivatization UV–Vis detection. Food Chemistry, 146, 353–362.
Durazzo, A., Kiefer, J., Lucarini, M., Camilli, E., Marconi, S., Gabrielli, P., Aguzzi, A., Gambelli, L., Lisciani, S., & Marletta, L. (2018). Qualitative analysis of traditional italian dishes: FTIR approach. Sustainability, 10(11), 4112.
Ferreira, M. P., Oliveira, M. C. N. de, Mandarino, J. M. G., Silva, J. B. da, Ida, E. I., & Carrão-Panizzi, M. C. (2011). Changes in the isoflavone profile and in the chemical composition of tempeh during processing and refrigeration. Pesquisa Agropecuária Brasileira, 46(11), 1555–1561.
Handoyo, T., & Morita, N. (2006). Structural and functional properties of fermented soybean (tempeh) by using Rhizopus oligosporus. International Journal of Food Properties, 9(2), 347–355.
Kurniadi, M., Arsyad, M. F., Sar, A. M., Nurhayati, R., & Wiyono, T. (2019). The effect of fermentation duration and starter concentration on physicochemical properties of modified sorghum flour by Lactobacillus plantarum FNCC 0027. Food Res, 3, 441–447.
Landau, S. Y., Dvash, L., Yehuda, Y., Muklada, H., Peleg, G., Henkin, Z., Voet, H., & Ungar, E. D. (2018). Impact of animal density on cattle nutrition in dry Mediterranean rangelands: a faecal near-IR spectroscopy-aided study. Animal, 12(2), 265–274.
Leopold, L. F., Leopold, N., Diehl, H.-A., & Socaciu, C. (2011). Quantification of carbohydrates in fruit juices using FTIR spectroscopy and multivariate analysis. Spectroscopy, 26(2), 93–104.
Lestari, O. A., & Mayasari, E. (2016). Potensi gizi tempe berbahan dasar jagung. Jurnal Ilmiah Teknosains, 2(2/Nov).
Lucarini, M., Durazzo, A., Kiefer, J., Santini, A., Lombardi-Boccia, G., Souto, E. B., Romani, A., Lampe, A., Ferrari Nicoli, S., & Gabrielli, P. (2020). Grape seeds: Chromatographic profile of fatty acids and phenolic compounds and qualitative analysis by FTIR-ATR spectroscopy. Foods, 9(1), 10.
McMullin, D., Mizaikoff, B., & Krska, R. (2015). Advancements in IR spectroscopic approaches for the determination of fungal derived contaminations in food crops. Analytical and Bioanalytical Chemistry, 407(3), 653–660.
Reyes-Moreno, C., Romero-Urias, C., Milán-Carrillo, J., Valdez-Torres, B., & Zárate-Márquez, E. (2000). Optimization of the solid state fermentation process to obtain tempeh from hardened chickpeas (Cicer arietinum L.). Plant Foods for Human Nutrition, 55(3), 219–228.
Rosmini, R., Arham, Z., & La Ode Anhusadar, N. (n.d.). Quality of Coconut Water Soy Sauce in the Presence of Powder Tempeh as an Additive.
Schön, A., Clarkson, B. R., Jaime, M., & Freire, E. (2017). Temperature stability of proteins: Analysis of irreversible denaturation using isothermal calorimetry. Proteins: Structure, Function, and Bioinformatics, 85(11), 2009–2016.
Song, S. Y., Lee, Y. K., & Kim, I.-J. (2016). Sugar and acid content of Citrus prediction modeling using FT-IR fingerprinting in combination with multivariate statistical analysis. Food Chemistry, 190, 1027–1032.
Suwanto, A., Rahayu, G., & Nuraida, L. (2013). Population dynamics of yeasts and lactic acid bacteria (LAB) during tempeh production. Hayati Journal of Biosciences, 20(2), 57–64.
Tahir, A., Anwar, M., Mubeen, H., & Raza, S. (2018). Evaluation of Physicochemical and Nutritional Contents in Soybean Fermented Food Tempeh by Rhizopus oligosporus. Journal of Advances in Biology & Biotechnology, 1–9.
Valdar, W. S. J., & Thornton, J. M. (2001). Protein–protein interfaces: analysis of amino acid conservation in homodimers. Proteins: Structure, Function, and Bioinformatics, 42(1), 108–124.
Van de Voort, F. R. (1992). Fourier transform infrared spectroscopy applied to food analysis. Food Research International, 25(5), 397–403.
Wycherley, T. P., Moran, L. J., Clifton, P. M., Noakes, M., & Brinkworth, G. D. (2012). Effects of energy-restricted high-protein, low-fat compared with standard-protein, low-fat diets: a meta-analysis of randomized controlled trials. The American Journal of Clinical Nutrition, 96(6), 1281–1298.
Yu, X., Zhao, M., Hu, J., Zeng, S., & Bai, X. (2012). Correspondence analysis of antioxidant activity and UV–Vis absorbance of Maillard reaction products as related to reactants. LWT-Food Science and Technology, 46(1), 1–9.
Zhang, W., Li, N., Feng, Y., Su, S., Li, T., & Liang, B. (2015). A unique quantitative method of acid value of edible oils and studying the impact of heating on edible oils by UV–Vis spectrometry. Food Chemistry, 185, 326–332.